ALLOANTIGEN-INDUCED T-HELPER ACTIVITY .1. MINIMAL GENETIC DIFFERENCES NECESSARY TO INDUCE A POSITIVE ALLOGENEIC EFFECT

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 120  (6) , 1897-1901
Abstract
Addition of histoincompatible lymphocytes can influence the course of ongoing immune responses. Such allogeneic effects may augment or diminish immune responses. The minimal genetic differences necessary to generate positive allogeneic effects (''allohelp'') in a humoral immune response were described. The antibody response to sheep erythrocytes of T [thymus-derived] cell-depleted mouse spleen cells was reconstituted by addition of syngeneic or allogeneic nylon wool column-passaged spleen T cells. T cells were pretreated with mitomycin C before culture to prevent development of allosuppression and cytotoxic lymphocytes. Positive allogeneic effects were operationally defined as superior helper effects (to generate greater antibody forming cell responses) with T cells allogeneic rather than syngeneic to the responding B [bone marrow-derived] cells. Addition of allogeneic T cells resulted in many more antibody forming cells than did equal numbers of syngeneic T cells, and fewer allogeneic than syngeneic T cells were necessary to generate comparable responses. With congenic, recombinant and mutant mouse lines, genetic differences in the H-2 complex and those associated with Mls were each sufficient to provide positive allogeneic effects. With intra-H-2 recombinants, differences at I or D were sufficient. A disparity at H-2K alone, as provided by the H-2 mutant B6.C-H-2ba against the parental line C57BL/6By, also induced helper effects.

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