A set of BAC clones spanning the human genome
Open Access
- 7 July 2004
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 32 (12) , 3651-3660
- https://doi.org/10.1093/nar/gkh700
Abstract
Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC clones spanning the human genome. The clone set provides coverage for at least 98% of the human fingerprint map, 99% of the current assembled sequence and has an effective resolving power of 79 kb. We have made the clone set publicly available, anticipating that it will generally facilitate FISH or array-CGH-based identification and characterization of chromosomal alterations relevant to disease.Keywords
This publication has 23 references indexed in Scilit:
- Integrated and Sequence-Ordered BAC- and YAC-Based Physical Maps for the Rat GenomeGenome Research, 2004
- A tiling resolution DNA microarray with complete coverage of the human genomeNature Genetics, 2004
- Software for Automated Analysis of DNA Fingerprinting GelsGenome Research, 2003
- DNA microarrays for comparative genomic hybridization based on DOP‐PCR amplification of BAC and PAC clonesGenes, Chromosomes and Cancer, 2003
- A Bacterial Artificial Chromosome Library for Sequencing the Complete Human GenomeGenome Research, 2001
- Integration of cytogenetic landmarks into the draft sequence of the human genomeNature, 2001
- A physical map of the human genomeNature, 2001
- Initial sequencing and analysis of the human genomeNature, 2001
- Human BAC Ends Quality Assessment and Sequence AnalysesGenomics, 2000
- Software for genome mapping by fingerprinting techniquesBioinformatics, 1988