A quantitative study of chiasma terminalization in the grasshopper Chorthippus jucundus

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Abstract
The possible existence of chiasma terminalization in the grasshopper Chorthippus jucundus was tested in four males by means of comparisons between chiasma locations at diplotene and metaphase I within L3, M4 and M5 bivalents. Diplotene cells were stained by a C-banding technique to recognize heterochromatic regions, especially the centromeric ones, whereas metaphase I cells were stained by a silver staining technique that visualizes a core-like structure that extends through each homologous chromosome. The core is very pronounced at kinetochores and forms cross-shaped configurations at chiasmata. No evidence of chiasma terminalization has been found.