Complete amino acid sequence of the neurone‐specific γ isozyme of enolase (NSE) from human brain and comparison with the non‐neuronal α form (NNE)

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Abstract
The complete amino acid sequence (433 residues) of the human neurone‐specific γ isozyme of enolase (NSE) has been determined by a combination of direct amino acid sequencing and nucleotide sequencing of cloned cDNA. Substantial amino acid sequence of the non‐neuronal α form of the enzyme was also obtained which agreed almost entirely with the indirect cDNA sequence [9]. Comparison of the two human sequences shows no insertions or deletions, but 72 replacements. Comparison of the human γ form with the corresponding isozyme from the rat shows only 7 replacements (compared to 27 changes between the human and rat α isozymes). We have identified regions of sequence difference between the human α and γ forms that are mainly hydrophilic in character (residues 271–285, 298–316 and 416–433). These residues are on the surface of the three‐dimensional structure [21] and could be useful as immunogens to produce antibodies specific for the neurone‐specific form.