LYSINE, METHIONINE AND TRYPTOPHAN CONTENT OF MICROORGANISMS I. Bacteria

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Abstract
The cells of 81 species of bacteria classified in 30 genera were analyzed for lysine and methionine (microbiological assay) and tryptophan (chemical procedure). The bacterial cells contained 2.6 to 13.5 (average, 6.5) g lysine, 0.7 to 4.5 (average, 1.8) g methionine, and 0.1 to 0.8 (average, 0.3) g tryptophan per 16 g cell nitrogen. In general, the quantities of these amino acids in the cells were not related to the classification of the organism. As much variation in the proportion of lysine in the cells occurred among species of the same genus as among the average values obtained for different genera. Eubacteriales had somewhat more lysine and methionine in their cells than did Actinomycetales; among the Eubacteriales, gram positive bacteria contained more lysine than did gram negative bacteria. The cells of organisms classified in the genera Bacillus, Micrococcus and Sarcina contained a significantly (P< 0.01) higher proportion of lysine than did those of other genera tested.

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