Rancidity in Lean Fish Muscle. V. The Effect of Amino Acids

Abstract
Addition of free amino acids to blended cod muscle affected the subsequent development of rancidity. In the presence of trace amounts of Cu++, the aromatic, heterocyclic, and sulphur-containing amino acids exerted varying degrees of antioxidant acitivity. In the absence of added metallic ions the aliphatic amino acids and cysteine showed strong pro-oxidant activity. Tests were carried out to determine the effect of pH and the concentration of amino acid on these reactions.Amino acid-induced rancidity was inhibited by the commercial antioxidants NDGA, PG, and BHT and by the chelating agent, EDTA; but not by tocopherol; ascorbic acid enhanced the oxidation. A limited number of tests indicated that the fish muscle did not undergo a seasonal variation in sensitivity to the amino acid-induced rancidity, and thus differed from the Cu++-induced rancidity.Those amino acids which inhibited metal-induced rancidities did not retard rancidity induced by the addition of sodium chloride.