Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein.

Open Access
Publisher Website
Google Scholar
Abstract
Mutation of the alpha‐actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha‐actinin gene. Transformants deficient in alpha‐actinin, an actin‐binding protein, produced an altered mRNA that lacked the 3′ portion of the coding region. The defect in alpha‐actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha‐actinin sequence and its complementary sequence in the coding region of the endogenous gene.