A COLORIMETRIC METHOD FOR SERUM CHYMOTRYPSIN DETERMINATION

1 February 1965

journal article
research article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry

Vol. 43 (2) , 135-141
https://doi.org/10.1139/o65-020

Abstract

A colorimetric method for serum chymotrypsin determination is presented. A specific substrate, N-acetyl-L-tyrosine hydrazide, is hydrolyzed by chymotrypsin, liberating hydrazine. This hydrazine is determined colorimetrically with Ehrlich's reagent. The procedure is suitable for the routine assay of serum chymotrypsin and its inhibitors.

Keywords

BLOOD CHEMICAL ANALYSIS
CATALYSIS
CHYMOTRYPSIN
COLORIMETRY
ENZYME INHIBITORS
HYDRAZINES
INDICATORS AND REAGENTS
KINETICS

This publication has 3 references indexed in Scilit:

A NEW COLORIMETRIC METHOD FOR SERUM TRYPSIN DETERMINATION
Canadian Journal of Biochemistry, 1964
Increased Plasma Proteolytic Activity Due to Arginine Amidase in Patients with Pancreatitis
New England Journal of Medicine, 1963
PROTEIN BINDING OF PANCREATIC PROTEOLYTIC ENZYMES*
Journal of Clinical Investigation, 1962