New RNA-protein crosslinks in domains 1 and 2 ofE.coli30S ribosomal subunits obtained by means of an intrinsic photoaffinity probe
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (4) , 1475-1491
- https://doi.org/10.1093/nar/17.4.1475
Abstract
Functionally active 705 ribosomes containing 4-thiouridine (s4U) in place of uridine were prepared by a formerly described in vivo substitution method. Proteins were crosslinked to RNA by 366nm photoactivation of s4U We observe the systematic and caracteristic formation of 30S dimers ; they were eliminated for analysis of RNA-protein crosslinks. M13 probes containing rDNA inserts complementary to domains 1 and 2 of 16S RNA from the 5end up to nucleotide 868 were used to select contiquous or overlapping RNA sections. The proteins covalently crosslinked to each RNA section were identified as S3, S4, S5, S7, S9, S18, S20 and S21. Several crosslinks are compatible with previously published sites for proteins S5, S18, S20 and S21 ; others for proteins S3, S4, S7, S9, S18 correspond necessarily to new sites.This publication has 17 references indexed in Scilit:
- RNA PROTEIN CROSSLINKS INTRODUCED INTO E. coli RIBOSOMES BY USE OF THE INTRINSIC PROBE 4-THIOURIDINEPhotochemistry and Photobiology, 1987
- Multiple crosslinks of proteins S7 and S9 to domains 3 and 4 of 16S ribosomal RNA in the Escherichia coli 30S particleEuropean Journal of Biochemistry, 1986
- Ribonucleic acid-protein crosslinking within the intact Escherichia coli ribosome, utilizing ethylene glycol bis[3-(2-ketobutyraldehyde) ether], a reversible, bifunctional reagent: identification of 30S proteinsBiochemistry, 1983
- Ribonucleic acid-protein crosslinking in Escherichia coli ribosomal 30S subunits by the use of two new heterobifunctional reagents: 4-azido-2,3,5,6-tetrafluoropyridine and 4-azido-3,5-dichloro-2,6-difluoropyridineBiochemical and Biophysical Research Communications, 1981
- Synthesis of a New Reagent, Ethyl 4‐azidobenzoylaminoacetimidate, and Its Use for RNA‐Protein Cross‐linking within Escherichia coli Ribosomal 30‐S SubunitsEuropean Journal of Biochemistry, 1980
- Stoichiometry of the 30S ribosomal proteins of Escherichia coliBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Ribosomal proteins of Escherichia coli. I. Purification of the 30 S ribosomal proteinsBiochemistry, 1969
- Association of rapidly labelled bacterial RNA with ribosomal RNA in solutions of high ionic strengthJournal of Molecular Biology, 1966
- THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITYBiochemical Journal, 1963