Identification of cell types in semithin epoxy sections of gastric fundic mucosa.

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Abstract
Procedures were developed to permit rapid and accurate identification of rat gastric cell types in epoxy-embedded fundic mucosa. The PAS[periodic acid-Schiff]-modified Bowie procedure resulted in magenta-stained secretory granules in the surface and pit mucoid cells. MNC [mucous neck cells], in contrast, had distinctly pink granules, while those of mature zymogen cells stained violet. Parietal cells were characteristically pale. The color of zymogen granules varied with other fixation techniques, from turquoise after glutaraldehyde alone, to very pale blue after osmium alone. Cell types were still easily distinguished. EM of adjacent thin sections confirmed identification of specific cells in the 1 .mu.m epoxy sections. The PAS-Bowie epoxy procedure may permit precise cell identification in autoradiographic studies of cellular proliferation and migration, without requiring prior removal of the epoxy resin.

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