A Rapid Method for Measuring Guanylate Cyclase Activity in Mammary Tissue

1 December 1975

journal article
research article
Published by Frontiers Media SA in Experimental Biology and Medicine

Vol. 150 (3) , 759-762
https://doi.org/10.3181/00379727-150-39120

Abstract

A simple and rapid method for measuring guanylate cyclase activity in broken cell preparations of biological tissues is described. This method employs the rate of conversion of [32P]GTP to [32P]cyclic-GMP. The product of this reaction is isolated by ion-exchange chromatography and by a ZnSO4-Ba(OH)2 precipitation at pH 5.7. Using this method, about 30-50 samples can be assayed for guanylate cyclase activity during a 5-6 h period. The characteristics of this enzyme in the mouse mammary gland were similar to those described for other tissues using different methods for measuring guanylate cyclase activity.

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