Autocrine growth stimulation of human meningioma cells by platelet-derived growth factor

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Abstract
✓ The authors have previously shown that meningioma-derived conditioned medium profoundly stimulates the in vitro proliferation of meningioma cells. In this paper, self-mitogenic agents found in the conditioned medium—autocrine growth-stimulatory factors actually secreted by human meningioma cells—are characterized as proteins related to the B chain of platelet-derived growth factor (PDGF) and possibly to the A chain of PDGF as well. The addition to conditioned medium of a neutralizing antibody against PDGF-BB caused a significant inhibition of the conditioned medium—stimulated DNA synthesis in all three meningioma cultures studied. A similar neutralizing effect was observed with an anti—PDGF-AA antibody in one meningioma culture studied. Gel filtration chromatography of concentrated conditioned medium from two different meningiomas using a Sephadex G-100 column revealed similar profiles from both conditioned media with a major peak of mitogenic activity against meningioma cells at a molecular weight (Mr)...