Divalent cation stimulation of in vitro fibre assembly from epidermal keratin protein
Open Access
- 1 October 1978
- journal article
- research article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 33 (1) , 255-263
- https://doi.org/10.1242/jcs.33.1.255
Abstract
Keratin was extracted from purified cornified cells of newborn rats in Tris-HCl-buffered 8 M urea containing beta-mercaptoethanol. Microfilaments were assembled in vitro by reducing the ionic strength of buffer and the urea concentration. One millimolar concentration of KCl and NaCl did not affect filament formation, but the same concentration of divalent cations greatly altered this process. CaCl2 and MgCl2 induced gelation of keratin by formation of bundles of birefringent macrofilaments. ZnCl2, CuSO4 and HgCl2 formed greater numbers of macrofilaments and the protein aggregated.This publication has 28 references indexed in Scilit:
- Self-assembly of bovine epidermal keratin filaments in vitroJournal of Molecular Biology, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Two polypeptide chain constituents of the major protein of the cornified layer of newborn rat epidermisBiochemistry, 1975
- Desmosomes, Filaments, And Keratohyaline Granules: Their Role In The Stabilization And Keratinization of The EpidermisJournal of Investigative Dermatology, 1975
- THE ROLE OF MICROFILAMENTS IN FROG SKIN ION TRANSPORTThe Journal of cell biology, 1973
- Isolation of Filaments from BrainScience, 1971
- Effect of calcium ion on S-100, a protein of the nervous systemBiochemistry, 1969
- The α-Fibrous Proteins of Epidermis**From the Departments of Dermatology and Orthopedic Research of the Harvard Medical School and the Massachusetts General Hospital Boston, Massachusetts 02114.Journal of Investigative Dermatology, 1968
- Cytoplasmic filaments and cell movements: Epidermal cells during ascidian metamorphosisJournal of Ultrastructure Research, 1966
- A nuclear ribonucleoprotein networkExperimental Cell Research, 1963