TRANSFORMATION TO PROTOTROPHY AND POLYGLUTAMIC ACID SYNTHESIS IN BACILLUS LICHENIFORMIS
- 1 July 1964
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 88 (1) , 220-+
- https://doi.org/10.1128/jb.88.1.220-225.1964
Abstract
Leonard, C. Gomez (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.), D. K. Mattheis, M. J. Mattheis, and R. D. Housewright . Transformation to prototrophy and polyglutamic acid synthesis in Bacillus licheniformis . J. Bacteriol. 88: 220–225. 1964.—Growth conditions necessary for transformation to prototrophy of 14 auxotrophs of Bacillus licheniformis were studied. The unexpected finding of different growth requirements by each auxotroph for the development of transformable cells is discussed. Under optimal growth conditions for a serine-deficient mutant transformation frequencies of 0.1% were obtained. A medium for transformation of competent cells in liquid suspensions is described. In addition, this report presents evidence for the transformation of three nonencapsulated mutants of B. licheniformis for the ability to synthesize polyglutamic acid (capsular material).Keywords
This publication has 19 references indexed in Scilit:
- Penetration of deoxyribonucleic acid into Hemophilus influenzaeBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1963
- Polyglutamic acid synthesis by cell-free extracts of Bacillus licheniformisBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1963
- Polyglutamic acid synthesis by cell-free extracts of Bacillus licheniformisBiochimica et Biophysica Acta, 1963
- Penetration of deoxyribonucleic acid into Hemophilus influenzae☆Biochimica et Biophysica Acta, 1963
- Transformability of Haemophilus influenzaeJournal of General Microbiology, 1962
- Molecular fate of DNA in genetic transformation of PneumococcusJournal of Molecular Biology, 1962
- Studies on Transformations of Hemophilus influenzae The Journal of general physiology, 1961
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961
- Isolation of d- and l-Glutamyl Polypeptides from Culture Filtrates of Bacillus subtilisJournal of Biological Chemistry, 1958
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956