Inhibition of TPA-induced NF-κB nuclear translocation and production of NO and PGE2 by the anti-rheumatic gold compounds

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Abstract
Auranofin, aurothioglucose and aurothiomalate (10 μM each) inhibited 12-O-tetradecanoylphorbol 13-acetate (TPA, 16.2 nM)-induced nuclear translocation of nuclear factor-kappa B (NF-κB), and production of nitric oxide (NO) and prostaglandin E2 (PGE2) in rat peritoneal macrophages when the cells were pre-incubated with each gold compound for 20h. Without pre-incubation for 20h, aurothioglucose and aurothiomalate, but not auranofin, failed to inhibit the TPA-induced NF-κB nuclear translocation and production of NO and PGE2. Auranofin, aurothioglucose and aurothiomalate did not affect the direct binding of NF-κB to the DNA probe. It was suggested that these gold compounds inhibit the TPA-induced production of NO and PGE2 by inhibiting the NF-κB nuclear translocation.

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