Development of a bioluminescence assay for aldehyde pheromones of insects
- 1 June 1982
- journal article
- research article
- Published by Springer Nature in Journal of Chemical Ecology
- Vol. 8 (6) , 911-921
- https://doi.org/10.1007/bf00987658
Abstract
A rapid, quantitative assay for long-chain aldehydes based on bacterial luminescence was developed. Significant luminescent responses were obtained with both saturated and unsaturated aldehydes of 12–18 carbons. Maximum responses were obtained with the 14- and 16-carbon compounds, including those that are known insect sex pheromones. The bioluminescent response was linearly related to the amount of aldehyde over a 104-105 concentration range with as little as 0.1 pmol (∼20 pg) of aldehyde being detected. The bioluminescent assay represents a new quantitative tool for rapidly measuring aldehyde pheromones of insects.Keywords
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