SANDWICH RADIOIMMUNOASSAY USING SINGLE MONOCLONAL-ANTIBODY THAT DETECTS MINUTE AMOUNTS OF MELANOMA ANTIGENS FROM VARIOUS MAMMALIAN-SPECIES

  • 1 January 1984
    • journal article
    • research article
    • Vol. 75  (5) , 427-432
Abstract
A sensitive sandwich radioimmunoassay for the detection of minute amounts of melanoma antigens from various mammalian melanomas was established by using a syngeneic mouse monoclonal anti-melanoma antibody (M2590). The assay system detected the soluble mouse and human melanoma antigens equally at a concentration of 103-104 cells/ml because the M2590 antibody reacted specifically with cross-species melanoma antigenic determinants composed of the carbohydrate side chains of the 31,000-dalton glycoprotein uniquely expressed on various mammalian melanoma cells. Human melanomas of various types, e.g., melanotic, amelanotic, primary, metastatic, etc., were successfully detected in an antigen-specific manner. Therefore, the present sandwich assay system using a single monoclonal antibody should be quite useful in clinical applications, especially in the diagnosis of human melanomas.

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