Calcium Localized in Odontogenic Cells of Rat Mandibular Teeth by the Glyoxal Bis (2-Hydroxyanil) Method

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Abstract
SYNOPSIS IN INTERLINGUA LE LOCALISATION DE CALCIUM IN CELLULAS ODONTOGENE DE DENTES MANDIBULAR DEL RATTO PER MEDIO DEL METHODO A GLYOXAL-BIS(2-HYDROXYANIL).—Blocos fresc de mandibula, de un spissitate de 1 mm, esseva obtenite ab rattos sugente e tincturate durante 16 horas in un solution de glyoxal-bis(2-hydroxyanil) (GBHA) e postea dishydratate in ethanol, acclarate in xyleno, includite in paraffin, sectionate a un spissitate de 7μ, e affixate per pression digital a albuminisate lamellas de vitro. Le lamellas esseva immergite in un solution de Na2CO3 e KCN pro assecurar specificitate pro calcium e contratincturate con blau methylenic. Granulos rubie de Ca-GBHA esseva localisate in le cytoplasma de adamantoblastos e cementoblastos e in le processos odontoblastic. Calcium in apatite non esseva tincturate.