Chemiluminescent labeled-antibody assay for thyroxin in serum, with magnetic separation of the solid-phase.
Open Access
- 1 March 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 32 (3) , 532-535
- https://doi.org/10.1093/clinchem/32.3.532
Abstract
A chemiluminescent labeled-antibody immunoassay has been developed for measurement of total thyroxin (T4) in serum. Monoclonal antibodies to T4 labeled with a chemiluminescent acridinium ester are used. Serum samples are incubated with the labeled antibodies and a thyroxin-rabbit immunoglobulin conjugate, then reacted with magnetizable particles containing sheep anti-rabbit immunoglobulin. The total reaction time is 40 min. The chemiluminescence intensity of the solid-phase immune complexes is inversely proportional to the concentration of T4 in the sample. The sensitivity of the assay is 1 nmol/L, and the working range of 20-190 nmol/L is characterized by CVs less than or equal to 10%.This publication has 5 references indexed in Scilit:
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