Removal of PCR Inhibitors from Forensic Samples and Enhanced Sensitivity for D1S80 using Fluorescent Scanning
- 1 January 1996
- journal article
- research article
- Published by Taylor & Francis in Canadian Society of Forensic Science Journal
- Vol. 29 (1) , 29-32
- https://doi.org/10.1080/00085030.1996.10757045
Abstract
It has been observed that PCR results may not always be obtained for forensic samples even though sufficient DNA appears to be present by slot blot. It has been proposed that this is the result of Taq polymerase inhibitors present in the samples. By using a combination of sample dilution, Thiopropyl Sepharose beads, BSA and additional Taq polymerase, it may be possible to overcome the inhibition of the polymerase. Although this treatment has been used with some success in our laboratory, it does not work with every sample. Substitution of “BLOTTO” in place of BSA has resulted in successful amplifications.Keywords
This publication has 1 reference indexed in Scilit:
- Attenuation of PCR inhibition in the presence of plant compounds by addition of BLOTTONucleic Acids Research, 1995