Use of 4-(6-methylnaphthalen-2-yl)-4-oxobut-2-enoic acid as a reagent for the spectrophotometric and fluorimetric determination of aliphatic thiol drugs
- 1 January 1988
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 113 (9) , 1447-1452
- https://doi.org/10.1039/an9881301447
Abstract
The aroylacrylic acid 4-(6-methylnaphthalen-2-yl)-4-oxobut-2-enoic acid is proposed as a useful reagent for the spectrophotometric and fluorimetric determination of aliphatic thiol compounds. Under mild reaction conditions (pH 7.4, room temperature) the compound reacts rapidly (reaction complete in 15 min) and selectively with thiol compounds to give stable fluorescent thiol adducts. The adducts can be determined in the presence of the reagent excess by spectrophotometric (conventional and second- or third-derivative procedures) and fluorimetric (λem.= 445 nm, λex.= 300 nm) methods. All the described procedures have been successfully applied to the determination of thiol drugs such as N-acetylcysteine, mercaptopropionylglycine and captopril in commerical dosage forms.Keywords
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