THE IMMUNOCHEMICAL SPECIFICITY OF ANTISERA TO BOVINE PARATHYROID HORMONE: AN APPROACH TO REGION-SPECIFIC RADIOIMMUNOASSAY

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Abstract
SUMMARY Six different antisera to bovine parathyroid hormone were characterized immunochemically by comparing their reactivity towards amino-terminal (1–29) and carboxyl-terminal (53–84) fragments of the hormone molecule. Three of the antisera were primarily amino-terminally directed. One antiserum contained antibody populations to at least three discrete sites; two other antisera each contained two antibodies directed against each end region of the hormone. By saturating one of these antisera with one or the other of the end fragments of the hormone, specific immunoassays for the amino-terminal and carboxyl-terminal regions of the molecule were developed. Such techniques may be applied to improving the specificity of the radioimmunoassay for parathyroid hormone by eliminating interference from carboxyl-terminal hormonal fragments which circulate in human plasma.

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