Potentiation by adrenaline of thrombin‐induced elevation of pHi is not essential for synergistic activation of human platelets
- 3 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 250 (2) , 211-214
- https://doi.org/10.1016/0014-5793(89)80722-7
Abstract
Gel-filtrated human platelets were stimulated with thrombin in the absence and presence of adrenaline. Adrenaline markedly enhanced the thrombin-induced increase in cytoplasmic pH (pHi) in BCECF-loaded platelets. This rise in pHi was strongly inhibited by the Na+/H+ exchange blocker EIPA. The potentiation by adrenaline of thrombin-induced PLC activation measured as [32P]PA formation and final platelet responses was, however, not blocked by EIPA, even at low concentrations of thrombin. These results indicate that the enhancement by adrenaline of thrombin-induced cytoplasmic alkalinization may be a secondary effect which is not essential for the potentiation by adrenaline of platelet activation by thrombin.Keywords
This publication has 17 references indexed in Scilit:
- Synergism between thrombin and adrenaline (epinephrine) in human platelets. Marked potentiation of inositol phospholipid metabolismBiochemical Journal, 1988
- The Na+H+ antiporter is not involved in potentiation of thrombin-induced responses by epinephrineBiochemical and Biophysical Research Communications, 1988
- The synergistic effect of serotonin and epinephrine on the human platelet at the level of signal transductionFEBS Letters, 1987
- Possible mechanisms of the potentiation of blood-platelet activation by adrenalineBiochemical Journal, 1987
- Activation of phospholipases A and C in human platelets exposed to epinephrine: role of glycoproteins IIb/IIIa and dual role of epinephrine.Proceedings of the National Academy of Sciences, 1986
- Synergistic responses in human platelets. Comparison between aggregation, secretion and cytosolic Ca2+ concentrationEuropean Journal of Biochemistry, 1986
- Platelet ADP receptor and alpha 2-adrenoreceptor interaction. Evidence for an ADP requirement for epinephrine-induced platelet activation and an influence of epinephrine on ADP binding.Journal of Biological Chemistry, 1986
- Changes in Cytoplasmic pH and in Membrane Potential in Thrombin-Stimulated Human PlateletsEuropean Journal of Biochemistry, 1981
- Epinephrine inhibits adenylate cyclase and stimulates a GTPase in human platelet membranes vis α‐adrenoceptorsFEBS Letters, 1981
- Novel α2-adrenoreceptors primarily responsible for inducing human platelet aggregationNature, 1979