LOCALIZATION OF A NUCLEAR ENVELOPE-ASSOCIATED PROTEIN BY INDIRECT IMMUNOFLUORESCENCE MICROSCOPY USING ANTIBODIES AGAINST A MAJOR POLYPEPTIDE FROM RAT-LIVER FRACTIONS ENRICHED IN NUCLEAR ENVELOPE-ASSOCIATED MATERIAL

  • 1 January 1978
    • journal article
    • research article
    • Vol. 18  (1) , 22-38
Abstract
The location of a specific major polyeptide present in nuclear pore complex-enriched fractions from rat liver was examined by indirect immunofluorescence microscopy using chicken antibodies against this polypeptide. In both whole cell preparations of cultured cells grown on cover slips (mouse 3T3 (embryo fibroblast), rat kangaroo kidney PtK2) and in frozen sections through liver and mammary gland tissue, a strongly preferential binding to the nuclear periphery of interphase cells was observed. The specificity of this localization was demonstrated in these cells by chromatin decoration with antibodies against histones and of elements of the endoplasmic reticulum (outer mitochondrial membrane) system with antibodies to cytochrome b5. The localization was examined by EM using frozen sections and immunoperoxidase techniques. This polypeptide is contained in a protein specific for the nuclear periphery, probably closely associated with the peripheral chromatin.