Greenhouse Technique for Evaluating Sweetclover Weevils’ Preference for Seedling Sweetclover Plants1

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Abstract
A method for evaluating preference of adult sweetclover weevils (sitona cylindricollis Fahr.) for seedling sweetclover plants is discussed. Seedling plants of a homozygous weevil-susceptible selection of Melilotus alba were subjected to the feeding of various numbers of adult weevils. Replicated tests were made with 1, 2, 4, and 6 weevils per plant. Statistical analyses run on the individual plant data showed significant differences between rows of plants. When assumed varieties were randomized so that each appeared the same number of times in each row, no significant differences were noted. Five varieties per flat replicated in two 5 × 5 Latin squares infested with two weevils per plant produced the most reliable results. The use of a homozygous susceptible standard check will provide, for flat to flat, and year to year, comparisons of materials tested.

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