Affinity labelling ofEscherichia coliribosomes with a benzylidene derivative of AUGU6within initiation and pretranslocational complexes
- 7 July 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 202 (2) , 340-344
- https://doi.org/10.1016/0014-5793(86)80714-1
Abstract
Affinity labelling of E. coli ribosomes with the 2',3'-O-[4-(N-2-chloroethyl)-N-methylamino]benzylidene derivative of AUGU6 was studied within the initiation complex (complex I) obtained by using fMet-tRNAfMet and initiation factors and within the pretranslocational complex (complex II) obtained by treatment of complex I with the ternary complex Phe-tRNAPhe-GTP-EF-Tu. Both proteins and rRNA of 30 S as well as 50 S subunits were found to be labelled. Sets of proteins labelled within complexes I and II differ considerably. Within complex II, proteins S13 and L10 were labelled preferentially. On the other hand, within complex I, multiple modification is observed (proteins S4, S12, S13, S14, S15, S18, S19, S20/L26 were found to be alkylated) despite the single fixation of a template in the ribosome by interaction of the AUG codon with fMet-tRNAfMetKeywords
This publication has 9 references indexed in Scilit:
- Direct cross-linking of heptauridilate to E. coli ribosomes by water-soluble carbodiimide in the complex stabilized by codon-anticodon interaction at both A- and P-sitesFEBS Letters, 1985
- The modelling of the decoding site of theEscherichia coliribosomeNucleic Acids Research, 1984
- Non‐exclusion principle of Ac‐Phe‐tRNAPhe interaction with the donor and acceptor sites of Escherichia coli ribosomesFEBS Letters, 1982
- The effect of poly(U) on the arrangement of tRNAPhe in donor tRNA‐binding site of Escherichia coli ribosomesFEBS Letters, 1982
- Quantitative study of the interaction of aminoacyl-tRNA with the a site ofEscherichia coliribosomesFEBS Letters, 1981
- The proteins of the messenger RNA binding site of Escherichia coli ribosomesNucleic Acids Research, 1981
- Mechanism of codon—anticodon interaction in ribosomesFEBS Letters, 1980
- Mechanism of codon-anticodon interaction in ribosomes. Direct functional evidence that isolated 30S subunits contain two Codon-specific binding sites for transfer RNANucleic Acids Research, 1980
- Crosslinking of chemically reactive A-U-G analogs to ribosomal proteins within initiation complexesJournal of Molecular Biology, 1979