Defined Single Strains of Lactic Streptococci in Bulk Culture for Cheddar and Monterey Cheese Manufacture

Abstract
Acid production and Cheddar cheese quality were improved at 3 cheese plants when undefined commerical blends of lactic cultures were removed and replaced with phage insensitive, single strains propagated either in pairs or in 1 multiple blend of 5 or 6 strains. The paired strain program worked in 1 plant for over 12 mo. with only 6 strains used during the last 7 mo. Two mutants replaced parent strains. The multiple blend worked for 6 mo. in 2 plants with only 1 mutant replacing a parent strain. Careful phage-activity monitoring and strain selection were necessary to assure optimal success of the programs. A refrigerated reserve culture was used to provide back-up for the primary blend in the plant using the paired strain program. Make-time and final cheese pH were more uniform than when undefined culture blends were used.