Search for Virus in Human Malignancies. 1. In vitro Studies.

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Abstract
Exhaustive investigations were made employing cell culture methods to recover virus from specimens from human cases of leukemia and other malignancies. Altogether, 316 clinical specimens were tested. Primary culture of the human malignant tissue, and passage of extracts of clinical specimens to primary cell cultures and to cultures of a cell strain were done. The specimens were tested crude and after treatment to remove hypothetical inhibitors, and the cultures were observed by a variety of procedures to detect virus by direct and indirect methods and before and after treatment to remove inhibitors. No detectable virus or virus-like agent of human malignant tissue origin was recovered. The finding of a simian virus multinucleating agent, MNA, emphasized the need for reliable controls and for caution in interpretation. The negative findings in these experiments were interpreted as examples of methods which failed and were not construed as positive evidence for a non-viral etiology of malignancies in man.
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