Alteration of the Carbohydrate-Binding Specificity of the Bauhinia purpurea Lectin through the Preparation of a Chimeric Lectin
- 1 January 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 111 (1) , 87-90
- https://doi.org/10.1093/oxfordjournals.jbchem.a123724
Abstract
A chimeric lectin gene was constructed by using a cDNA clone coding the Bauhinia purpurea lectin (BPA) in which a part of the metal-binding region was replaced by the corresponding region of the mannose-bindlng Lens culinaris lectin (LCA). The chimeric lectin expressed in Escherichia coli was found to bind α mannosyl-bovine serum albumin (BSA) and this binding was inhibited by mannose.Keywords
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