c‐Myc‐mediated expression of nucleophosmin/B23 decreases during retinoic acid‐induced differentiation of human leukemia HL‐60 cells
Open Access
- 16 November 2004
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 578 (3) , 211-216
- https://doi.org/10.1016/j.febslet.2004.08.089
Abstract
The retinoic acid‐induced differentiation of human leukemia HL‐60 cells towards mature granulocytic cells was accompanied by the decline in the protein levels of c‐myc, nucleophosmin/B23 and its promoter activity. These RA‐induced effects were further enhanced by the concurrent treatment of HL‐60 cells with p38 map kinase inhibitor SB203580 (SB). It seems that there is a strong correlation of nucleophosmin/B23 and c‐Myc expressions in cells under RA treatment. Furthermore, nucleophosmin/B23 promoter activity decreased upon c‐Myc antisense‐mediated reduction of intracellular amount of c‐Myc. CHIP assays showed that binding of c‐Myc to the nucleophosmin/B23 promoter decreased in RA‐treated cells. Thus, nucleophosmin/B23 expression is targeted by c‐Myc during RA‐induced differentiation. These results provide evidence for a novel mechanism of transcriptional downregulation of nucleophosmin/B23 and the functional role of c‐Myc in RA‐induced differentiation.Keywords
This publication has 30 references indexed in Scilit:
- UV Stimulation of Nucleophosmin/B23 Expression Is an Immediate-early Gene Response Induced by Damaged DNAJournal of Biological Chemistry, 2002
- Over-expression of nucleophosmin/B23 decreases the susceptibility of human leukemia HL-60 cells to retinoic acid-induced differentiation and apoptosisInternational Journal of Cancer, 2000
- Down-regulation of nucleophosmin/B23 during retinoic acid-induced differentiation of human promyelocytic leukemia HL-60 cellsOncogene, 1998
- Identification and characterization of nucleophosmin/B23/numatrin which binds the anti-oncogenic transcription factor IRF-1 and manifests oncogenic activityOncogene, 1997
- Suppression of c-myc or fosB-induced cell transformation by the transcription factor IRF-1Cancer Letters, 1994
- Fusion of a Kinase Gene, ALK , to a Nucleolar Protein Gene, NPM , in Non-Hodgkin's LymphomaScience, 1994
- ADP‐ribosylation of nucleolar proteins in HeLa tumor cellsJournal of Cellular Biochemistry, 1993
- Characterization and cellular localization of nucleophosmin/B23 in HeLa cells treated with selected cytotoxic agents (studies of B23-translocation mechanism)Experimental Cell Research, 1992
- Nuclear Substrates of Protein Kinase CEuropean Journal of Biochemistry, 1992
- The nuclear matrix protein, numatrin (B23), is associated with growth factor-induced mitogenesis in Swiss 3T3 fibroblasts and with T lymphocyte proliferation stimulated by lectins and anti-T cell antigen receptor antibody.The Journal of cell biology, 1988