Abstract
Periodic acid-Schiff (PAS) applied to histological sections of ovinemuscles, infected naturally with sarcocysts, showed that only macroscopic “fat” Sarcocystis tenella had PAS-positive secondary cyst-walls after fixation only. Macroscopic “thin” S. medusiformis and microscopic S. ovicanis sarcocysts had cyst-walls that were not positive despite fixation.

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