Isolation and Characterization of a Mouse Protein C cDNA
- 1 April 1992
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 111 (4) , 491-495
- https://doi.org/10.1093/oxfordjournals.jbchem.a123785
Abstract
Protein C (PC) i8 a vitamin K-dependent serine protease, a deficiency of which results In thrombus. There Is no spontaneously occurring mouse model of the disease. Attempts to create such a model in mice by using anti-sense gene technology requires Isolation of a normal mouse PC cDNA. When a mouse liver (BALB/c) cDNA library was screened using a human PC cDNA as a probe, nine overlapping cDNA clones were isolated and sequenced. The cloned mouse PC cDNA comprised 1,512 nucleotldes and the open reading frame of the cDNA encoded a polypeptide of 4431 amino acids residues including a leader peptide composed of 41 amino acids. Mouse PC exhibited high homology to both human and bovine PCs. Mouse PC also had several structural features common in other PCs; locations of 23 Cys residues, location of putative β-hydroxyAsp71, possible carbohydrate attachment sites involving Asp residues at amino acid positions 249,314, and 330, and location of active sites such as His212, Asp258, and Ser361. Northern blot hybridization analysis identified a single species of mouse PC mRNA (2.0 kb in length) In mouse liver.Keywords
This publication has 0 references indexed in Scilit: