Cytotonic effect of Clostridium difficile enterotoxin on Chinese hamster ovary cells

1 April 1986

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 34 (2) , 241-244
https://doi.org/10.1016/0378-1097(86)90361-7

Abstract

The effect of purified enterotoxin produced by Clostridium difficile on Chinese hamster ovary (CHO) cells was examined. In a certain concentration range (0.9–3.6 μ g/ml), purified toxin caused CHO elongation, namely a cytotonic effect, which is similar to a typical morphological change in CHO cells induced by cholera enterotoxin. At a higher concentration, purified enterotoxin had a cytotoxic effect on CHO cells which was neutralized by anti-C. difficile enterotoxin, but not by anti-C. difficile cytotoxin. Thus, enterotoxin had both cytotonic and cytotoxic effects on CHO cells. About 60 and 180 min were required for binding of enterotoxin to CHO cells, and its internalization, respectively, both times being much longer than those for C. difficile cytotoxin.

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