Ca2+uptake and IP3-induced Ca2+release in permeabilized human lymphocytes
- 5 October 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 222 (2) , 349-352
- https://doi.org/10.1016/0014-5793(87)80400-3
Abstract
The 45Ca2+ uptake and 45Ca2+ release in saponin-permeabilized human lymphocytes were studied. An ATP-dependent Ca2+ uptake into a nonmitochondrial, intracellular Ca2+ store is observed which is approx. 2 orders of magnitude greater than the ATP-independent Ca2+ uptake. The Ca2+ uptake is inhibited by vanadate, but it is insensitive to oligomycin and ruthenium red. IP3 induces dose-dependent 45Ca2+ release. For half-maximum Ca2+ release 0.25–0.5 μM IP3 is required. The results of our studies suggest that 45Ca2+ is predominantly stored within the endoplasmic reticulum of the lymphocytes.Keywords
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