Specific Localization In Vivo of Antihepatoma Antibodies in Autochthonous Rat Hepatomas2

Publisher Website
Google Scholar
Abstract
Radioiodinated antihepatoma antibodies, after purification in vivo from induced hepatomas in rats, localized in the total hepatic region (liver with hepatoma) of hepatoma-bearing rats to an extent as high as 60 percent of the injected dose. The localized antibodies were shared almost equally by hepatoma and non-neoplastic portions. When the purified antibodies were passively transferred through normal rats, cross-localizing antibodies were absorbed from the circulation. Antibodies that remained in the serum, when assayed in hepatoma-bearing rats, preferentially localized in discrete hepatomas and localized to as high as 40 percent of the injected dose in the total hepatic region. When assayed in normal rats, the same preparation localized at the 4 percent level in liver. Antihepatoma antibodies, when purified in vivo from normal liver of normal rats and assayed in hepatoma-bearing rats, preferentially localized in the non-neoplastic portions of the hepatic region.