Separation and Purification of Liver Microsomal Monooxygenases from Induced and Untreated Pigs

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Abstract
From liver microsomes of phenobarbital-, .beta.-naphthoflavone- and ethanol-pretreated male pigs and of controls NADPH-cytochrome P-450 reductase and 4 cytochrome P-450 fractions were isolated according to a modified procedure of Imai and Sato. Electrophoretic, immunological, spectral and catalytic properties suggested that at least 5 different forms of cytochrome P-450 could be separated, 2 of which were obtained in a pure state. Using selective inhibitors and antisera it was concluded that microsomes from phenobarbital-pretreated pigs contain at least 3 forms which were all metyrapone-sensitive. .beta.-Naphthoflavone probably induced two 7,8-benzoflavone-sensitive forms. After ethanol administration the main form of cytochrome P-450 was identical to that of controls.