Towards a Systematic Validation of References in Real-Time RT-PCR
Open Access
- 29 July 2008
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 20 (7) , 1734-1735
- https://doi.org/10.1105/tpc.108.059774
Abstract
Real-time RT-PCR (also known as quantitative RT-PCR [qRT-PCR]) is a powerful tool for quantifying gene expression, combining both high sensitivity and specificity with efficient signal detection. It has relatively recently begun to be used to monitor gene expression in plants (reviewed in [Gachon etThis publication has 15 references indexed in Scilit:
- The lack of a systematic validation of reference genes: a serious pitfall undervalued in reverse transcription‐polymerase chain reaction (RT‐PCR) analysis in plantsPlant Biotechnology Journal, 2008
- Normalisation of real-time RT-PCR gene expression measurements in Arabidopsis thaliana exposed to increased metal concentrationsPlanta, 2008
- The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycleThe Plant Journal, 2007
- Quantification of mRNA using real-time RT-PCRNature Protocols, 2006
- Real-time PCR: what relevance to plant studies?Journal of Experimental Botany, 2004
- Real‐time RT‐PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root‐ and shoot‐specific genesThe Plant Journal, 2004
- Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlationsBiotechnology Letters, 2004
- Heat-stress-dependency and developmental modulation of gene expression: the potential of house-keeping genes as internal standards in mRNA expression profiling using real-time RT-PCRJournal of Experimental Botany, 2003
- Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genesGenome Biology, 2002
- Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCRNucleic Acids Research, 2002