Molecular Genetic Analysis of AMP‐dependent Protein Kinase
- 1 October 1986
- journal article
- review article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 478 (1) , 162-174
- https://doi.org/10.1111/j.1749-6632.1986.tb15529.x
Abstract
Genetic evidence has been obtained indicating that the responsiveness of CHO cells to cAMP requires an intact cADepPK system. DNA carrying mutant RI and C subunit genes has been transferred to wild-type cells. Recipient cells carrying the DNA have been detected by selecting for expression of the phenotype for cAMP resistance. We are in the process of cloning a functional mutant RI subunit to use as a moveable genetic element that can confer cAMP resistance on recipient cells. This cloned gene should allow us to test hypotheses concerning the mechanism of cAMP regulation of transcription in mammalian cells.Keywords
This publication has 36 references indexed in Scilit:
- DNA-mediated transfer of cAMP resistance in CHO cellsJournal of Cellular Physiology, 1986
- The phosphoform of the regulatory subunit RII of cyclic AMP-dependent protein kinase possesses intrinsic topoisomerase activityCell, 1985
- Amino acid sequence of the regulatory subunit of bovine type I cAMP-dependent protein kinaseBiochemistry, 1984
- Genetic evidence that a phorbol ester tumor promoter stimulates ornithine decarboxylase activity by a pathway that is independent of cyclic AMP‐dependent protein kinases in CHO cellsJournal of Cellular Physiology, 1982
- Localization of the catalytic subunit of cyclic AMP-dependent. Protein kinase in cultured cells using a specific antibody.The Journal of cell biology, 1982
- Genetic approaches to cyclic AMP effects in cultured mammalian cellsCell, 1980
- Regulation of transglutaminase activity in Chinese hamster ovary cellsBiochimica et Biophysica Acta (BBA) - General Subjects, 1980
- Evidence suggesting that a cyclic AMP‐dependent protein kinase is a positive regulator of proliferation in Cloudman S91 melanoma cellsJournal of Cellular Physiology, 1979
- Properties of protein kinase and adenylate cyclase‐deficient variants of a macrophage‐like cell lineJournal of Cellular Physiology, 1979
- Isolation of mutant adrenocortical tumor cells resistant to cyclic nucleotidesMolecular and Cellular Endocrinology, 1977