Multiple roles for the yeast SUB2/yUAP56 gene in splicing
Open Access
- 1 January 2001
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 15 (1) , 36-41
- https://doi.org/10.1101/gad.852101
Abstract
The UAP56 gene has been shown to be required for prespliceosome assembly in mammals. We report here the isolation of theSchizosaccharomyces pombe ortholog of this gene by heterologous complementation of a combinedPRP40HA 3/nam8Δ defect in budding yeast. The Saccharomyces cerevisiae ortholog,YDL084w/SUB2, is also able to suppress this defect. We show that SUB2 is involved in splicing in vivo as well as in vitro. Sub2 defective extracts form a stalled intermediate that contains U2snRNP and can be chased into functional spliceosomes. Our experiments also suggest a role for this protein in events that precede prespliceosome formation. Data reported here as well as in the accompanying papers strongly implicate Sub2p in multiple steps of the spliceosome assembly process.Keywords
This publication has 26 references indexed in Scilit:
- A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicingThe EMBO Journal, 2000
- An RNA Switch at the 5′ Splice Site Requires ATP and the DEAD Box Protein Prp28pMolecular Cell, 1999
- Disruption of sixSaccharomyces cerevisiae genes from chromosome IV and basic phenotypic analysis of deletion mutantsYeast, 1998
- U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction.Genes & Development, 1997
- Identification and characterization of a yeast homolog of U1 snRNP-specific protein CThe EMBO Journal, 1997
- The Saccharomyces cerevisiae Prp5 Protein Has RNA-dependent ATPase Activity with Specificity for U2 Small Nuclear RNAJournal of Biological Chemistry, 1996
- Identification of Prp40, a Novel Essential Yeast Splicing Factor Associated with the U1 Small Nuclear Ribonucleoprotein ParticleMolecular and Cellular Biology, 1996
- A novel role for a U5 snRNP protein in 3' splice site selection.Genes & Development, 1995
- New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiaeYeast, 1994
- Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicingCell, 1989