Ultrastructural Localization of K+-Dependent, Ouabain-Sensitive Nppase (Na-K-Atpase) In the Guinea Pig Inner Ear
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Acta Oto-Laryngologica
- Vol. 95 (1-4) , 277-289
- https://doi.org/10.3109/00016488309130944
Abstract
Ultrastructural localization of the phosphatase component of Na-K-ATPase in the inner ear of the guinea pig was studied utilizing p-nitrophenyl phosphate (NPP) as substrate. In the stria vascularis NPPase activity was restricted to the cytoplasmatic side of the basal plasma membranes of the marginal cells. In the spiral prominence enzyme activity was resolved only occasionally on the basolateral membranes of the spiral prominence epithelium, especially after longer incubation periods. The remaining inner ear tissue was unreactive. Possible transport mechanisms of potassium across the apical marginal cell membrane are discussed.Keywords
This publication has 16 references indexed in Scilit:
- Reliability and specificity of membrane adenosine triphosphatase localizations.Journal of Histochemistry & Cytochemistry, 1980
- Replacement of perilymph with K+-free media abolishes positive endocochlear potential (guinea pig)The Journal of the Acoustical Society of America, 1979
- Further Studies of the Membrane Potential of the Stria Cells of the Guinea PigIn VitroActa Oto-Laryngologica, 1979
- Transport ATPase cytochemistry: ultrastructural localization of potassium-dependent and potassium-independent phosphatase activities in rat kidney cortex.The Journal of cell biology, 1975
- THE CYTOCHEMICAL APPLICATION OF NEW POTENT INHIBITORS OF ALKALINE PHOSPHATASESJournal of Histochemistry & Cytochemistry, 1973
- TRANSPORT ADENOSINE TRIPHOSPHATASE CYTOCHEMISTRY I. BIOCHEMICAL CHARACTERIZATION OF A CYTOCHEMICAL MEDIUM FOR THE ULTRASTRUCTURAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT PHOSPHATASE ACTIVITY IN THE AVIAN SALT GLANDJournal of Histochemistry & Cytochemistry, 1972
- Studies on (Na+-K+)-activated ATPase XXIV. Localization and properties of ATPase in the inner ear of the guinea pigBiochimica et Biophysica Acta (BBA) - Biomembranes, 1969
- Observations on the electrochemistry of the cochlear endolymph of the rat: a quantitative study of its electrical potential and ionic composition as determined by means of flame spectrophotometryProceedings of the Royal Society of London. B. Biological Sciences, 1968
- Evaluation of adenosine triphophatase activity in the stria vascularis and spiral ligament of normal guinea pigs.The Laryngoscope, 1967
- The Coarse Pattern of the Electrical Resistance in the Cochlea of the Guinea Pig (Electroanatomy of the Cochlea)The Journal of the Acoustical Society of America, 1951