Effect of Copulation Time on Spermatozoan Transfer of Diabrotica virgifera (Coleoptera: Chrysomelidae)1

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Abstract
The percent of Diabrotica virgifera females receiving a spermatophore and the percent of females containing spermatozoa in the spermathecae increased directly with duration of copulation. A prolonged copulatory period of ca. 3–4 h was necessary for maximal insemination of female D. virgifera during a single copulation. Spermatophores of mated females gradually degenerated and disappeared 7 days after mating. Spermatozoa were present and remained viable within the spermathecae up to 40 days following mating.

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