Method for Salmonella concentration from water at pH 3.5, using micro-fiber glass filters

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Abstract
A method is described for the concentration of Salmonella from water. As is done with enterovirus, Salmonella bacteria were concentrated from water in 2 steps: by pH 3.5 adsorption on and pH 9.5 elution from 8 .mu.m porosity micro-fiber glass filter tubes. This method worked in less than 30 min; S. typhimurium was inactivated only slightly in spite of rapid pH variations (pH 3.5-9.5). The retention by the filters stems from 2 phenomena: a low retention in the micro-fiber glass labyrinth for small filtered volumes and a high retention by adsorption at pH 3.5 for any filtered volume (experiments done with 15- and 80 l samples). Addition in tap water of trivalent ions like Al3+ did not increase Salmonella adsorption. In most trials, Salmonella recovery varied from 42-93%. Preliminary field investigations indicate that enterovirus and Salmonella may both be concentrated from the same water sample by this procedure.