Proton magnetic resonance spectra of tRNAfMetfrom Thermus thermophilus
- 1 March 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (3) , 585-593
- https://doi.org/10.1093/nar/4.3.585
Abstract
220 MHz proton magnetic resonance spectra of tRNAs in bulk and tRHAfMet from Thermus therwophilus have been measured and compared with those of tRNAs from E. coli. Temperature dependences and chemical shift positions of the bulk tRNAs are well explained by the difference in their GC contents. It is known that the base sequence of the double helical regions in the cloverleaf structure of T. thermophilus tRNAfMet is different from that of E. coli tRNAfMet only at two positions in TψC arm; one more C:G pair is contained instead of a U:G pair of E. coll tRNAfMet and a C:G pair of E. coli is replaced by a G:C pair. In spite of the resembrance in the base sequences, nmr patterns around 13 ppm are fairly different from each other. The difference is discussed in relation with their tertiary structures and with the origin of chemical shift displacements.Keywords
This publication has 27 references indexed in Scilit:
- Changes in tertiary structure accompanying a single base change in transfer RNA. Proton magnetic resonance and aminoacylation studies of Escherichia coli tRNAMetf1 and tRNAMetf3 and their spin-labeled (S4U8) derivativesBiochemistry, 1976
- CD spectra of 5-methyl-2-thiouridine in tRNAfMet from an extreme thermophileNucleic Acids Research, 1976
- NMR evidence for tertiary structure base pair in E. coli tRNA involving S4U8Nature, 1974
- Assignment of the Hydrogen Bonded Proton Resonances in ( Escherichia coli ) tRNA Glu by Sequential MeltingProceedings of the National Academy of Sciences, 1974
- Biological Sciences: Model for the Secondary Structure of the Denatured Conformer of Yeast tRNA3LeuNature, 1974
- Ring-current shifts in the 300 MHz nuclear magnetic resonance spectra of six purified transfer RNA moleculesJournal of Molecular Biology, 1973
- Determination of Secondary and Tertiary Structural Features of Transfer RNA Molecules in Solution by Nuclear Magnetic ResonanceProceedings of the National Academy of Sciences, 1973
- Assignment of the low field proton nuclear magnetic resonance spectrum of yeast phenylalanine transfer RNA to specific base pairsJournal of Molecular Biology, 1973
- Investigation of exchangeable protons and the extent of base pairings in yeast phenylalanine transfer RNA by high resolution nuclear magnetic resonanceJournal of Molecular Biology, 1972
- High Resolution Nuclear Magnetic Resonance Studies of Hydrogen Bonded Protons of tRNA in WaterNature, 1971