Enhanced Sensitivity to Camptothecin in Ataxia-telangiectasia Cells and Its Relationship with the Expression of DNA Topoisomerase I

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Abstract
The antitumor drug camptothecin (CPT) can trap covalently bound topoisomerase I-DNA intermediates as complexes which conceal single-strand scissions. In an attempt to evaluate the cytotoxic potential of these lesions in human cells we have measured: (1) cell cycle delay and cell killing by CPT in primary and transformed fibroblasts, and in lymphoblastoid lines derived from normal, X-ray sensitive ataxia-telangiectasia (A-T) and xeroderm pigmentosum (XP) donors; (2) the properties of sublines obtained by high-dose selection of CPT: (3) levels of drug-induced DNA strand scission in intact cells; (4) the cellular availability of extractable topoisomerase I. The drug induced a marked cell cycle block in G2 phase, the magnitude of the block being closely related to cell kill. XP group A cells showed normal sensitivity to CPT, whereas A-T derived cells were consistently hypersensitive (3-5 fold) in a manner which could not be related to a primary deficiency in topoisomerase I activity, abnormal capacity for complex formation or anomalies in the intracellular generation of DNA strand breaks. A CPT-resistant A-T subline had reduced topoisomerase I activity but retained the characteristic of hypersensitivity to X-radiation. The subline lost resistance upon in vitro passage with evidence that resistance was initially an unstable feature of a subpopulation of cells. The findings have implications for the role of topoisomerase I in the in vitro phenotype of A-T cells, and the contribution made by topoisomerase I-dependent damage to the cytotoxic action of CPT.