Synthesis of Lens Protein in vitro: Formation of beta-Crystallin
Open Access
- 1 September 1977
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 78 (2) , 617-622
- https://doi.org/10.1111/j.1432-1033.1977.tb11775.x
Abstract
Upon addition of lens polyribosomes to a reticulocyte-cell-free system, α, βl, and γ crystallin are synthesized, while βh crystallin is not formed. This phenomenon is comparable to the bio-synthetic events in the lens-cell-free system and in tissue culture. It is shown that βh crystallin formation depends upon the presence of a polypeptide βB1b which arises by posttranslational modification. The putative precursor for βB1b is a polypeptide βB1a of which the messenger with a sedimentation coefficient of 12.5 S has been isolated.This publication has 12 references indexed in Scilit:
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