A Revised Tannin-Osmium Method for Non-Coated Scanning Electron Microscope Specimens

1 January 1974

journal article
Published by International Society of Histology & Cytology in Archivum histologicum japonicum

Vol. 36 (3) , 189-193
https://doi.org/10.1679/aohc1950.36.189

Abstract

A revision in preparation technique for non-coated scanning electron microscope specimens is reported. Use of arginine hydrochloride, glycine, sodium glutamate and sucrose before treatment with tannic acid markedly enhanced the osmium staining of biological specimens and enabled direct scanning electron microscope observation at magnification as high as 30, 000×. The advantages of this method were discussed.

Keywords

OSMIUM
GLYCINE
MARKEDLY
SODIUM
TREATMENT
REVISED
COATED
SCANNING ELECTRON MICROSCOPE