A NOVEL MAST-CELL GROWTH-FACTOR (MCGF-3) PRODUCED BY MARROW-ADHERENT CELLS THAT SYNERGIZES WITH INTERLEUKIN-3 AND INTERLEUKIN-4

  • 1 August 1990
    • journal article
    • research article
    • Vol. 18  (7) , 794-800
Abstract
A clonal marrow-adherent stromal cell line, +/+-1 LDA11, was derived and found to produce hemopoietic stimulatory activity for an interleukin 3 (IL-3)-dependent mast cell line, NFS/N1. This factor-dependent mast cell line displayed restricted growth factor responsiveness to only IL-3, interleukin 4 (IL-4), and the stromal cell-produced factor. The factor produced by stromal cells was distinguished from IL-3 and IL-4 and was characterized biochemically. This factor appears to be a novel mast cell growth factor (MCGF- 3) capable of synergizing with IL-3 and IL-4. It may have broader reactivity in hemopoiesis than simply IL-3-dependent mast cells, and it may prove relevant to stromal cell-mediated hemopoiesis.