Determination of the Free Thyroxine Content of Serum

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Abstract
A simple method for determining the free thyroxine content of serum is presented. It involves the in vitro labeling of the total serum thyroxine with thyroxine-I131 and separation of the free from protein-bound fractions on microcolumns of Sephadex G-25. Relating the radioassay values for the free thyroxine to the specific activity of the protein-bound moiety permits calculation of the free thyroxine content. This method was used in a series of normal subjects and those with a variety of thyroid abnormalities; results in the latter group were consonant with the thyroid status.

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