2-Amino Sugar-containing Oligosaccharides Isolated from Hydrazinolyzate of Blood Group A Mucopolysaccharide of Hog Gastric Mucus
- 1 January 1962
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 51 (1) , 1-11
- https://doi.org/10.1093/oxfordjournals.jbchem.a127493
Abstract
Blood group A mucopolysaccharide prepared from hog gastric mucus was hydrazinolyzed, and the 2-amino sugar-containing substances which were obtained after acid hydrolysis of the hydrazinolyzed product were N-acetylated. Fractionation of the N-acetylated 2-amino sugar-containing compounds on cellulose powder columns and charcoal columns resulted in isolation of two crystalline oligosac charides: Oligosaccharides I and II. Oligosaccharides I and II were characterized and the structure of these compounds has been fully established on the basis of the analytical data, the values of sodium metaperiodate oxidation and their physical properties: Oligosaccharide I is O-β-2-acetamido-2-deoxy-D-glucopyranosyl-(1→-3)-D-galactose and Oligosaccharide II is O-α-2-acetamido-2-deoxy-D-galactopyranosyl-(1→3)-D-galactose.Keywords
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