Process monitoring by parallel column gradient elution chromatography
- 1 June 1991
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 63 (11) , 1114-1118
- https://doi.org/10.1021/ac00011a011
Abstract
A system was developed that generates two gradients simultaneously, 180 degrees out of phase. Essential elements of the system were a pump delivering solvent A at a constant velocity, a pump delivering solvent B at a constant velocity, two mixing chambers, and a ten-port valve. By use of this gradient device and two reversed-phase columns packed with 1000-A pore diameter, 8-microns particle size macroporous poly-(styrene-divinylbenzene) resin, six proteins were separated in 40 s in a single gradient cycle.Keywords
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